arac - Mar 4 2017 You are correct sepd in thinking that since the translation of mRNA begins with AUG which codes for methionine then all proteins should contain a methionine at their Nterminus aka start site Do restriction enzymes on read 339 to 539 Biology Stack Exchange Do all proteins start with methionine Biology Stack Exchange Newest 39plasmids39 Questions Biology Stack Exchange On which strand does the promoter sit Biology Stack Exchange The AraC gene can activate GFP expression because the GFP in the pGLO plasmid has the negative inducible pBAD promoter In the absence of arabinose an AraC protein dimer binds the promotor region but does not activate transcription When arabinose is present the protein binds in a different way which allows the downstearm gene here GFP to Jan 6 2014 The arti objektif answer to this question depends upon the definition of the word 39promoter39 In the simplest possible model of prokaryotic transcription the promoter is the site where RNA polymerase binds to the DNA before initiating RNA synthesis What is the purpose of the AraC gene in pGLO Stack Exchange Network Stack Exchange network consists of 183 QA communities including Stack Overflow the largest most trusted online community for developers to learn share their knowledge and build their careers If the AraC protein was a gene repressor when it binds arabinose would there be high or low transcription levels when arabinose is present closed If the AraC protein were to function as a gene repressor when it binds arabinose would there be high or low transcription levels filosofi sepatu when arabinose is present
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