pacuh - Versatile protein tagging in cells with berkulit split fluorescent protein pACUHGFP110 sec Addgene pACUH 10X UAS version of pACU 10X UAS hsp70 promoter5UTR SV40 early polyA recommended for intermediate expression Addgene 58374 download Sapar et al 2018 pACU2 high expression with HIH cassette 5X UAS hsp70 promoter5UTR synthetic intron His2Av polyA possible leaky expression induced by enhancers near the insertion site Plasmid pACUHGFP110 sec from Dr Daichi Kamiyamas lab contains the insert GFP110 sec and is published in Proc Natl Acad Sci U S A 2021 Jun 811823 pii 2024690118 doi 101073pnas2024690118 This plasmid is available through Addgene With free online banking checking and savings accounts Piedmont Advantage Credit Union has great financial services in North Carolina Become a member Plasmid pACUH from Dr YuhNung Jans lab is published in Cell Rep 2018 Aug 2824922732286 doi 101016jcelrep201807095 This plasmid is available through Addgene Image Illustrated plasmid map in PNG format GenBank File Plasmid sequence and annotations Use text editor or plasmid mapping software to view sequence PACU North Carolina Credit Union Checking Savings Accounts For the expression of GFP110 GFP110 was synthesized and cloned into pACUH vectors pcDNA31 vectors as well as modified lentiviral pHRSFFV vector 32 For GFP110 parat sequence information see PDF pACUH mediaaddgeneorg Share your videos with friends family and the world Plasmid pACUHGFP11x7mCherryαtubulin from Dr Bo Huangs lab contains the insert GFP11x7mCherryαtubulin and is published in Nat Commun 2016 Mar 18711046 doi 101038ncomms11046 This plasmid is available through Addgene We fused these colour variants to the N terminus of Drosophila βactin and cloned them into pACUH vectors To prepare GFP11 repeat arrays constructs we first synthesized GFP11 x1 x3 or x7 with long 15 aa or short 5 aa linkers see the sequence information in Supplementary Table 2 Then these GFP11 fragments were fused to βtubulin pACUH Addgene 58374 product information labomecom pACUH catalog 58374 more info or order Addgene product webpage citations 4 Reference Banzai K Shen P Kamiyama D A Genetic Toolkit for Simultaneous Generation of LexA and QFExpressing Clones in Selected Cell Types in Drosophila Neurosci Insights 20221726331055211069939 pubmed publisher Versatile protein tagging in cells with split fluorescent protein Nature pACUH Addgene Pacuh YouTube pACUH All sites shown are unique Han lab plasmid map pACUH P3 05162014 Title Print File Author Thinkpad Created Date 5282008 32511 PM pACUHGFP11x7mCherryαtubulin Addgene Han Lab Drosophila Transgenic Vectors The kode situs porno Han Lab Cornell University
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