pnpp - paraNitrophenylphosphate pNPP is a nonproteinaceous chromogenic terminal kalideres substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays 1 Phosphatases catalyze the hydrolysis of pNPP liberating inorganic phosphate and the conjugate base of paranitrophenol pNP The resulting phenolate is yellow with a maximal absorption at 405 nm 2 chromogenic 990 enzymatic powder or crystals CAS Number 333338184 EC Number 2242465 Synonyms pNitrophenyl phosphate disodium hexahydrateDisodium 4nitrophenyl phosphate hexahydratediSodium 4nitrophenyl phosphatepNPP disodium hexahydrate Linear Formula O2NC6H4OPOONa2 6H2O at SigmaAldrich PNPP pNitrophenyl Phosphate Disodium Sal is a widely used substrate for detecting alkaline phosphatase in ELISA applicationsWhen alkaline phosphatase and PNPP are reacted a yellow watersoluble reaction product is formed This reaction product absorbs light at 405 nm PNPP Paranitrophenyl phosphate disodium is a nonproteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays In Vitro Phosphatases catalyze the hydrolysis of PNPP liberating inorganic phosphate and the conjugate base of paranitrophenol pNP 4Nitrophenyl phosphate pnitrophenyl phosphate pNPP is widely used as a small molecule phosphotyrosinelike substrate in activity assays for protein tyrosine phosphatases It is a colorless substrate that upon hydrolysis is converted to a yellow 4nitrophenolate ion that can be monitored by absorbance at 405 nm Therefore the pNPP assay paraNitrophenylphosphate Wikipedia KGold Substrate is a onebottle stabilized chromogenic substrate for use with alkaline phosphatase angka hijaiyah 1-30 based immunoassays KGold Substrate utilizes the substrate pnitrophenylphosphate PNPP which reacts with alkaline phosphatase to produce the chromophore pnitrophenol pNitrophenyl phosphate pNPP is the substrate of choice for use with alkaline phosphatase in enzyme linked immunosorbent assay ELISA procedures It is the substrate of choice in alkaline phosphatase systems due to its high sensitivity This substrate produces a soluble end product that is yellow in color and can be read pNitrophenyl phosphate C6H4NO6P2 CID 4686862 structure chemical names physical and chemical properties classification patents literature biological PNPP disodium Paranitrophenyl phosphate disodium Phosphatases 4Nitrophenyl phosphate disodium salt hexahydrate MilliporeSigma 4Nitrophenyl phosphate disodium salt hexahydrate MilliporeSigma PNPP is a nonspecific substrate for protein alkaline and acid phosphatases It is used to measure phosphatase activity by spectrophotometric assay based on the formation of pnitrophenol Structural analysis of human dualspecificity phosphatase 22 complexed pNitrophenyl Phosphate PNPP NEB KGold PNPP Substrate Diagnostics Neogen pNPP ELISA Substrate STEMCELL Technologies PNPP Substrates Thermo Fisher Scientific pNPP paranitrophenyl phosphate is a chromogenic substrate for alkaline phosphatase ALP that is widely used in ELISA assays pNPP ELISA Substrate is for use with STEMCELLs ELISA antibody pair kits and is supplied in a readytouse format in a diethanolamine buffer The reaction of pNPP ELISA Substrate with ALP results in a yellow water pNitrophenyl phosphate bangkauni C6H4NO6P2 CID 4686862 PubChem
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