spr2 - Plantspecific microtubuleassociated protein SPIRAL2 is

Brand: spr2

spr2 - Mar 5 2018 SPR2 function is bebana required to facilitate the rapid reorientation of plant cortical arrays as stimulated by light perception a process that is driven by microtubule severing to create a new The Arabidopsis SPIRAL2 Protein Targets and Stabilizes The SPR2 gene encodes a plantspecific 94kD protein containing HEATrepeat motifs that are implicated in proteinprotein interaction When expressed constitutively SPR2green fluorescent protein fusion protein complemented the spr2 mutant phenotype and was localized to cortical microtubules as well as other mitotic microtubule arrays in Who We Are SP2 helps schools and businesses reduce risk and instill cultures of safety in industries such as automotive service collision repair refinish heavydutydiesel welding construction cosmetology culinary and more SPR2 protects minus ends to promote severing and Jan 16 2018 The spr22 and ktn12 mutants and the construction of P spr2SPR2GFP were described previously Shoji et al 2004 Yao et al 2008 Nakamura et al 2010 spr22 and P spr2SPR2GFP transgenic lines Arabidopsis Col0 background were crossed with Col035SmCherryTUA5 andor YFPTUA5expressing lines Shaw et al 2003 Gutierrez et al Dec 29 2023 Two SPR2 alleles with a righthanded twisting growth phenotype spr24 and tor110 have TDNA insertions that cause frameshifts at SPR2 residues 627 and 630 respectively 13 14 These insertions compromise proper translation of the SPR2 Cterminal region residues 649864 highlighting the importance of the Cterminal region in SPR2 Mar 19 2018 Live imaging using variableangle epifluorescence microscopy 4 showed that SPR2mRuby localizes to multiple regions on cortical microtubulesIn agreement with previous reports 3 5 we detected SPR2mRuby signal along the length of cortical microtubules as mobile particles of varying intensities at microtubule crossover sites and occasionally at the growing and shortening plus ends of SPR2 protects minus ends to promote severing and Jul 1 2003 Using a mapbased cloning approach we demonstrate here that Spr2 encodes a chloroplast fatty acid desaturase involved in JA biosynthesis Loss of Spr2 function reduced the 183 content of leaves to 10 of wildtype levels abolished the accumulation of hexadecatrienoic acid and caused a corresponding increase in the level of dienoic fatty acids SPR2 protects minus ends to promote severing and Mar 19 2018 Fan et al show that SPIRAL2 SPR2 localizes at the acentrosomal cortical microtubule plus and minus ends and modulates their dynamics in an opposing manner thus influencing the lifetime of microtubule crossovers and severing frequency In vitro reconstitution experiments show that SPR2GFP binds stably to minus ends and inhibits their dynamics Arabidopsis SPIRAL2 promotes uninterrupted microtubule growth Plantspecific microtubuleassociated protein SPIRAL2 is The Arabidopsis SPIRAL2 protein targets and stabilizes Jul 15 2008 SPIRAL2 SPR2 of Arabidopsis thaliana is a microtubuleassociated protein containing multiple HEAT repeats that are found only in the plant lineage We show that SPR2 and SP2L their closest Arabidopsis homolog are expressed in various tissues with partially overlapping patterns and spr2sp2l dou Tomato Suppressor of prosysteminmediated responses2 Gene SP2 Safety Pollution Prevention Training Jul 15 2008 SPIRAL2 SPR2 of Arabidopsis thaliana is a microtubuleassociated protein containing multiple HEAT repeats that are found only in the plant lineage We show that SPR2 and SP2L their closest Arabidopsis homolog are expressed in various tissues with partially overlapping patterns and spr2sp2l double mutants exhibit enhanced righthanded helical growth Fusion to green fluorescent protein SPIRAL2 Determines Plant Microtubule skta Organization by Aug 17 2022 The endosperm in spr21 was clearly underdeveloped compared with that of the WT Figure 1 IN These results indicate that the Spr2 mutation arrests the embryo and endosperm development in maize SPR2 is targeted to mitochondria Spr2 is an intronless gene encoding a 35kDa SPR2 protects minus ends to promote severing and PubMed Mar 5 2018 Here we identify Arabidopsis thaliana SPIRAL2 SPR2 as a protein that tracks minus ends and protects them against subunit loss SPR2 function is required to facilitate the rapid reorientation of plant cortical arrays as stimulated by light perception a process that is driven by microtubule severing to create a new population of microtubules Crystal structure of the Arabidopsis SPIRAL2 Cterminal Oct 27 2022 Null mutations of Spr2 severely impair the splicing of 15 out of the 22 mitochondrial Group II introns resulting in substantially decreased mature transcripts which abolished the assembly and activity of mitochondrial complex I Consequently embryogenesis and endosperm development were arrested in the spr2 mutants Arabidopsis SPIRAL2 promotes uninterrupted microtubule growth The small PPR protein SPR2 interacts with PPRSMR1 to The Arabidopsis SPIRAL2 Protein Targets and Stabilizes Oct 7 2013 Consistent with the idea that mutation in spr21 results in fewer complex MT crossovers the proportion of simple crossovers involving two MTs in bot17spr21 double mutants 048 was approximately twice that found in bot17 single mutants 026 Figure 4 B although these differences were not statistically significant These data support an Mar 3 2018 For live imaging of SPR2 the pSPR2SPR2mRuby construct was generated using 2 kb sequence upstream of the SPR2 start codon and the full length SPR2 cDNA followed by the mRuby cDNA This construct was introduced into a pGAG plasmid for Agrobacteriummediated floral dip transformation Transgenic plants were selected using 100mgL gentamycin The Arabidopsis SPIRAL2 Protein Targets and Stabilizes Apr 16 2020 Metabolic heatmap generated with the 100 most intense ions detected in roots of control wildtype WT and spr2 mutant tomato plants or in roots of mycorrhizal WT WTM and spr2 spr2M plants sampled at 32 and 45 dpi Ions in aqueous methanol root extracts were detected by DLIESIMS as described in Materials and Methods The small PPR protein SPR2 interacts with PPRSMR1 to Aug 17 2022 Reciprocal crosses between spr21 and spr22 heterozygotes produced approximately a quarter emp mutant kernels Figure 1D indicating that Spr2 is the causative gene for the emp phenotype Reverse transcriptionPCR RTPCR analyses indicated that the WT Spr2 transcripts could not be detected in the spr2 alleles Figure 2B suggesting SPR2mRuby signal appeared as a dot that tracked the slowly depolymerizing minus ends The minusend localization of SPR2 is reminiscent of the metazoan CAMSAPPatroninNezha family of minusendtargeting proteins 10 However SPR2 is unrelated to this protein family and lacks their signature CKK domain which mediates microtubule minusend Mar 3 2018 Extinction is more likely in WT than spr22 because it is less deep into the unbounded growth regimen For this reason spr22 WT is the least likely to go extinct coupling a slowly shrinking minus end less crossover erasure and hence more amplification to strongly unbounded plusend growth large intrinsic survival probability The small PPR protein SPR2 interacts with PPRSMR1 to Distinct gene expression and frostek secondary metabolite profiles in

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Rp21.000
Rp436.000-892%
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