subab - Jan 9 2016 Depletion of subAB traficbun gene in STEC O113H21 impairs stress granule formation in Caco2 cells To determine whether STEC O113H21produced SubAB is an essential factor for SG formation we used a wildtype strain one lacking the subAB gene subAB strain and a subAB strain complemented with a wildtype SubAB expressing plasmid subABsubAB Subtilase cytotoxin SubAB is a AB5 type toxin produced by Shigatoxigenic Escherichia coli which exhibits cytotoxicity to Vero cells SubAB B subunit binds to toxin receptors on the cell surface whereas the A subunit is a subtilaselike serine protease that specifically cleaves chaperone BiPG Nov 19 2021 KLHDC7B is involved in SubABinduced apoptosis As shown by RNAseq analysis KLHDC7B mRNA was most significantly stimulated by SubAB Table 1First we tested if SubAB treatment increases Subtilase cytotoxin from Shigatoxigenic Escherichia coli SubAB is a serine protease produced by some Shiga toxigenic E coli strains that causes haemolytic uraemic syndrome It specifically targets and inactivates BiP an essential ER chaperone leading to cell death Subtilase cytotoxin from Shigatoxigenic Escherichia coli Subtilase cytotoxin produced by locus of enterocyte PubMed Subtilase cytotoxin from Shigatoxigenic Escherichia coli Subtilase cytotoxin produced by Shigatoxigenic Escherichia SubAB a specifically deadly toxin Nature Reviews Microbiology Sutab is a prescription laxative used by adults to clean the colon before a colonoscopy so the healthcare provider can see the inside of the colon more clearly Sutab can cause serious side effects including changes in certain blood tests heart problems seizures and ulcers of the bowel or bowel problems ischemic colitis Sep 9 2013 Intracellular trafficking of SubAB mutants Vero cells were treated with 3 μg ml 1 purified wild type SubAB or the indicated mutant derivatives for 30 min and then fixed permeabilized and differentially stained for SubA red or SubB green using immunofluorescence as described under Experimental Procedures Sutab for Conoscopy Prep Side Effects Dosage Interactions Mar 1 2017 SubAB is a toxin produced by some Shiga toxigenic Escherichia coli strains that can cause hemolytic uremic syndrome This study shows that SubAB causes ascites heart hypertrophy liver damage and kidney injury in rats with potential implications for HUS pathogenesis SubAB was delivered to the endoplasmic reticulum ER via a COG conserved oligomeric GolgiRab6COPI coat protein Idependent pathway In the ER SubAB cleaves a specific site at Leu416 on endoplasmic reticulum chaperone BiPGRP78 SubABdependent BiP cleavage is inhibited by brefeldin A BFA a Golgi complexdisrupting agent 15 16 A novel endoplasmic stress mediator Kelch domain Nature More than just sugars COG complex deficiency causes Structural Basis of Subtilase Cytotoxin SubAB Assembly Nov 1 2006 SubAB is the prototype member of a new class of AB 5 toxins and has a serine protease activity that is essential for cytotoxicity Paton and coworkers used a proteomics approach to search for data SubAB reduced cell death as evaluated by the lactate dehydrogenase LDH release assay Figure S1E These findings suggest that SubAB inhibits pyroptotic cell death in macrophages by caspase11 inhibition through GSDMD cleavage suppression SubAB prevents LPSinduced proIL1b expression by the PERK and promina crunchies IRE1adependent pathways SubAB a protease cleaves a specific site on the endoplasmic reticulum ER Autophagy and apoptosis play critical roles in cellular homeostasis and survival Subtilase cytotoxin SubAB produced by nonO157 type Shigatoxigenic Escherichia coli STEC is an important virulence factor in disease Nov 1 2006 The structure of SubAB and the accompanying functional characterization of various mutants of SubAB provide a framework for understanding the important role of the Bpentamer in the assembly and Apr 15 2022 SubAB is a toxin produced by some Shigatoxigenic Escherichia coli STEC strains that cleaves the ER chaperone BiP and triggers ER stress SubAB also suppresses inflammasome activation and IL1βIL18 production by inhibiting STAT1 and caspase11 expression which may help STEC survive and cause disease in hosts TOXINS SubAB a specifically deadly toxin Nature Feb 21 2022 The subtilase cytotoxin SubAB belongs to the family of AB5 toxins and is produced together with Shiga toxin Stx by certain Stxproducing E coli strains STEC For most ABtype toxins it is assumed that cytotoxic effects can only be induced by a complete holotoxin complex consisting of SubA and SubB However it has been shown for SubAB that the enzymatically active subunit SubA without DAP1 a Negative Regulator of Autophagy Controls SubAB Systemic effects of Subtilase cytotoxin produced by Escherichia coli Subtilase Cytotoxin MDPI Toxins SubAB a specifically deadly toxin ResearchGate The new allelic variant of the subtilase cytotoxin subAB2 SubAB toxin binds to the plasma membrane and then traffics through the Golgi to the ER where it cleaves target protein GRP78 In this assay SubAB toxin is added to cells for different time points then cell lysates are assessed for cleavage of GRP78 indicating that the toxin has reached ER via retrograde trafficking Figure 7A DAP1 a negative regulator of autophagy controls SubAB Subtilase cytotoxin SubAB is mainly produced by locus of enterocyte effacement LEEnegative strains of Shigatoxigenic Escherichia coli STEC SubAB cleaves an endoplasmic reticulum ER chaperone BiPGrp78 leading to induction of ER stress This stress causes activation of ER stress sensor p Dec 1 2009 SubAB is a cytotoxin produced by some STEC strains that cause haemolytic uraemic syndrome It consists of a subtilaselike serine protease SubA and a glycanbinding subunit SubB that trigger endoplasmic reticulum stress and apoptosis in eukaryotic cells Novel Aspects of the SubA Subunit of the Subtilase Cytotoxin Subtilase cytotoxin produced by locus of enterocyte Mar 10 2022 Subtilase cytotoxin SubAB is an AB 5 toxin mainly produced by the locus of enterocyte effacementnegative Shigatoxigenic Escherichia coli STEC strain such as O113H21 yet the contribution of SubAB to STEC infectious disease is unclear We found that SubAB reduced activation of the STEC O113H21 infectioninduced noncanonical NLRP3 Oct 25 2013 Subtilase cytotoxin SubAB is an AB 5 toxin produced by Shiga toxin Stxproducing Escherichia coli STEC strains usually lacking the eae gene product intimin Two allelic variants of SubAB encoding genes have been described subAB 1 located on a plasmid and subAB 2 located on a pathogenicity island PAI x'mas together with tia gene
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